The Kranj unit of the National Veterinary Institute (NVI) carries out professional, operational and development activities, which include microbiological and chemical testing of food and milk, microbiological testing of water, parasitological testing of faeces and diagnostics of animal infectious diseases, sampling of food and feed in the field, and activities in the field of bee healthcare.
Users of the unit’s services are veterinary inspectors, holders and breeders of animals, and producers and processors of food of animal origin.
The Kranj NVI strives to achieve the highest quality of laboratory services, hence in 2002 it obtained accreditation from the Faculty of Veterinary Medicine for testing laboratories according to the SIST EN ISO/IEC 17025 standard. The Annex to the LP-021 Accreditation Certificate, including a list of accredited methods, is available on the website of the Slovenian Accreditation Board.
Based on a decision by the Director General of the Directorate for Food Safety, Veterinary and Plant Protection, the Faculty of Veterinary Medicine was designated as a National Reference Laboratory (NRL) for milk, within which the unit operates. The tasks of the NRL are defined in section 60 of the Veterinary Compliance Criteria Act.
Expertise in analytics:
- carrying out courses for the purpose of sampling raw milk
- on the basis of agreement various training courses are carried out for agricultural holders
- various training courses for beekeepers
The unit also offers the opportunity for veterinary medicine students to do work experience and other such related orientations.
Unit employees are involved in research work in the field of laboratory diagnostics and the healthcare of bees within different programme groups. Research on milk and milk products is carried out within the NRL laboratory.
Microbiological and chemical examinations of milk are carried out for the purpose of controlling the quality of milk, meeting the criteria for raw milk in accordance with Regulation (EC) No.853/2004 and for the purpose of milk production control. Most of the following methods are ISO 17025 accredited:
- process of determining the total number of microorganisms using the plate counting method at 30°C (ISO 4833).
- process for counting bacteria in milk using a Bactoscan FC fluoro-opto-electronic instrument (ISO 21187)
- procedure for determining somatic cells in milk using a fluoro-opto-electronic apparatus (ISO 13366-2)
- determination of milk fat, protein, lactose and freezing point content in milk using an IR spectrometer (IDF 141C)
- procedure for determining the freezing point of milk using a cryoscope (ISO 5764)
- determination of inhibitory substances in milk - DELVO test
Non-accredited methods are also carried out, including:
- rapid test for the determination of aflatoxin M1 in milk using an immunochromatographic test
- rapid test for the determination of antibiotics in milk using an immunochromatographic test
- determination of alkaline phosphatase in milk (ISO 11816-1)
Determination of the acidic level of milk:
Within the NRL a conversion equation is calculated for the purposes of converting the automatic method units into units of the reference method for the territory of Slovenia.
Commission Regulation (EC) No. 2073/2005 applies in the EU, which sets uniform microbiological criteria for food. It mainly includes microorganisms, which are the most common causes of foodborne infections.
In the laboratory the following examinations are performed:
- horizontal method for the detection of Salmonella spp. according to ISO 6579-1
- tests to determine the presence of Salmonella spp. using a modified IRIS method
- tests to determine the presence and number of listeria monocytogenes according to ISO 11290.
- determination of β glucuronidase-positive Escherichia coli bacteria, according to ISO 16649-2
- determination of the total number of microorganisms according to ISO 4833
- tests to determine the presence of staphylococcal positive coagulase according to ISO 6888-1
- tests for the presence of enterobacteriaceae according to ISO 21528-2
- tests under the Food Safety Guidelines: bacillus cereus, pseudomonas, moulds and yeasts…
The process of determining alkaline phosphatase in cheese (ISO 11816-2) is also carried out, which is intended to determine whether the milk from which the cheese is made has been pre-treated.
Microbiological examinations are intended for the control of general hygienic conditions or cleanliness in establishments (equipment, space). To this end, examinations of surface swabs and carcasses are carried out, specifically:
- presence of Listeria monocytogenes
- presence of Salmonella spp.
- presence of enterobacteria
- total number of microorganisms
The microbiological suitability of water is determined using membrane filtration. The presence of faecal enterococci, E. coli and coliforms is determined in samples of drinking water as well as the total number of microorganisms.
With a view to detecting salmonella at all relevant stages of production, in particular at the level of primary production - including feed - in accordance with Regulation (EC) No.2160/2003 the Kranj NVI also implements the horizontal method for the detection of Salmonella spp. in excrement, cloths and other environmental samples, according to ISO 6579-1:2017.
In accordance with Regulation 2015/1375 on the official control of Trichinella in meat, procedures are carried out for the examination for the presence of Trichinellae spp.
The presence of eggs or larvae of internal parasites is detected in the faeces of domestic animals or larvae of internal parasites.
Investigations are conducted to identify the cause of mastitis and to test for antibiotic susceptibility. The key to sampling is the correct way of sampling. For this purpose, a video has been recorded at the Institute for Ruminants at the Faculty of Veterinary Medicine, which is available on this link.
Healthcare of bees
For the purpose of healthcare of bees, the prevalence of certain bee diseases are determined. In addition to clinical examinations performed by a specialist in bee healthcare, which are carried out directly in apiaries, laboratory tests for the following pathogens are carried out:
- nosema disease
- acarine mites
- European foulbrood
- chal brood
- small hive beetle
Veterinary Hygiene Service
The unit has an organised veterinary hygiene service, where, in the area it covers, it removes dead animals and transports them to the cadaver collection point and takes care of their harmless destruction. From the point of view of preventing the spread of the disease to both animals and humans, this service is of the utmost importance as removal eliminates the source of potential infection. It is also an important aspect of protecting the environment, as removing dead animals prevents water, soil and feed contamination.
NVI Unit Kranj
Kranjska cesta 16,
Tel.: +386 4 2770600
Fax.:+386 4 2770 618