General Data

Project description

The main objectives of the project are genotype-phenotype association study in Krškopolje pig, genetic variability assessment of Krškopolje pig population at genomic level (the distribution and frequency of structural polymorphisms) and a comparison of modern breed and fatty Krškopolje pig at epigenetic, metabolic and product (meat, fat) qualitative level including the study of the response of both breeds to different amounts of protein in the diet.

Partner UL-VF (together with KIS) takes part in the experiments including a comparison between a modern breed selected for leanness and a fatty local breed Krškopolje pig in epigenetics, metabolism, and product quality (meat, fat). Partner UL-VF is responsible for gene expression analyses and characterization of the tissues (skeletal muscle, fat) using histological and immunohistochemical analyses. Gene expression analysis of subcutaneous fat tissue and muscles will be performed using selected candidate genes approach and via sequencing RNA (RNA-seq) on the pooled samples. Histological samples will be analysed for metabolic and contractile status of skeletal muscle and lipids in fat and muscle tissue will be histomorphometrically assessed.