pes

Gensko posredovana terapija s plazmidom za IL-12 v zdravljenju spontanih tumorjev psov

lukam — Thu, 26 May 2022 14:31:52 +0000

Gene electrotransfer treatment with plasmid encoding IL-12 for spontaneous tumours in dogs lukam Thu, 05/26/2022 - 17:03 Klinika za male živali rak genska terapija elektrokemoterapija genski elektroprenos pes onkologija IL-12 Research

Advanced treatments for spontaneous tumors - better prospects for animals and valuable experience for human oncologists.

Gene electrotransfer of immuno stimulating cytokines are one of the advanced therapies, aimed at stimulating the patient's immune response against cancer.

Numerous clinical studies over the years have confirmed that this novel treatment is safe for both the patient and the environment and that has a positive effect on local tumour control and disease progression. The gene gene electrotransfer of immunostimulating cytokines provides the patient with a good quality of life as the side effects are minimal. This was the main reason why the owners found this treatment method suitable and would choose it again if needed.

We have published the results of our research at numerous scientific and professional meetings and in major internationally recognized journals, and we have also endeavored to share our achievements with the public. To get a glimpse of our work and results to date, we invite you to read some of the papers at the links below:

Simpozij o genski terapiji v Sloveniji - SAZU
Pitamic S. Slovenska platforma za razvoj genske terapije, Medicina danes, marec 2022
Čemažar M, T Jesenko, M Bošnjak in soavtorji. Genska terapija v onkologiji, prvi razvojni koraki v Slovenij. Onkologija, junij 2022

Authors: prof. dr. Nataša Tozon (Univerza v Ljubljani, Veterinarska fakulteta), dr. Nina Milevoj (Univerza v Ljubljani, Veterinarska fakulteta), dr. Urša Lampreht Tratar (Onkološki inštitut Ljubljana), prof. dr. Maja Čemažar (Onkološki inštitut Ljubljana)

Primerjava mezenhimskih matičnih celic iz maščobnega tkiva psov in mačk

lukam — Mon, 25 Jan 2021 09:51:53 +0000

Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells lukam Mon, 01/25/2021 - 10:55 Institute of Preclinical Sciences mesenchymal stem cells dog cat comparison proliferation differentiation cell surface marker viability Research

Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential.

Remarkable immunomodulatory abilities of mesenchymal stem cells, also called multipotent mesenchymal stromal cells or medicinal signaling cells (MSCs), have entailed significant advances in veterinary regenerative medicine in recent years. Despite positive outcomes from MSC therapies in various diseases in dogs and cats, differences in MSC characteristics between small animal veterinary patients are not well-known. We performed a comparative study of cells' surface marker expression, viability, proliferation, and differentiation capacity of adipose-derived MSCs (ADMSCs) from dogs and domestic cats. The same growth media and methods were used to isolate, characterize, and culture canine and feline ADMSCs. Adipose tissue was collected from 11 dogs and 8 cats of both sexes. The expression of surface markers CD44, CD90, and CD34 was detected by flow cytometry. Viability at passage 3 was measured with the hemocytometer and compared to the viability measured by flow cytometry after 1 day of handling. The proliferation potential of MSCs was measured by calculating cell doubling and cell doubling time from second to eighth passage. Differentiation potential was determined at early and late passages by inducing cells toward adipogenic, osteogenic, and chondrogenic differentiation using commercial media. Our study shows that the percentage of CD44+CD90+ and CD34−/− cells is higher in cells from dogs than in cells from cats. The viability of cells measured by two different methods at passage 3 differed between the species, and finally, canine ADMSCs possess greater proliferation and differentiation potential in comparison to the feline ADMSCs. The results show that in the development of new cell therapies it is necessary to take into account the animal species and the results of stem cell research cannot be easily transferred from one species to another.

Authors: Metka Voga, Valerija Kovač, Gregor Majdič

The full article is published in Frontiers in Veterinary Science

Nitrozativni stres v možganih psov s pasjo kognitivno motnjo

lukam — Mon, 18 Jan 2021 14:28:53 +0000

Nitrosative stress in brains from dogs with canine cognitive dysfunction lukam Mon, 01/18/2021 - 15:47 Institute of Preclinical Sciences Kognitivna motnja pes nitrozativni stres nNOS eNOS iNOS 3-nitrotirozin Research

Nitrosative stress in the frontal cortex from dogs with canine cognitive dysfunction.

Canine cognitive dysfunction (CCD) is an age-related disorder similar to human Alzheimer’s disease (AD) that occurs in elderly dogs. Nitrosative stress has been implicated as one of the causes leading to neurodegenerative diseases, particularly AD. Its involvement in the development of CCD has not been studied so far. In the present study, immunohistochemical staining detected all three isoforms of nitric oxide synthases (nNOS, eNOS, and iNOS) and 3-nitrotyrosine (3-NT) in brains from CCD-affected dogs and non-demented control dogs in all layers of the canine frontal cortex. In CCD-affected and non-demented brains, nNOS was highly expressed in pyramidal-like neurons in the upper cortical layers. nNOS has also been observed in astrocytes in the CCD frontal cortex. The nNOS immunohistochemical staining was statistically significantly elevated in dogs with CCD in comparison to non-demented dogs. Blood vessel wall cells were positive for eNOS, which was also expressed in astrocytes and neurons. Intense 3-NT immunoreactivity was observed in the upper cortical layers, where amyloid-beta deposits spread in the last stage of CCD. Brain cells in the same area were highly
immunoreactive for iNOS. This infers that neuroinflammation and nitrosative stress might exacerbate the neurodegenerative process in CCD-affected brains, ultimately leading to cognitive impairment.

Slika 1. nNOS protein v pasjih možganih. Slika prikazuje značilno obarvane živčne celice (rjavo) v čelnem režnju 16 letnega shih-tzuja s pasjo kognitivno motnjo. Merili sta dolgi 50 µm.

Slika 2. 3-NT v pasjih možganih. 3-NT (zeleno obarvanje) je prisoten v celicah, ki obkrožajo amiloide (Aβ) plake (rdeče obarvanje) pa tudi izven celic v območju plaka. Celična jedra so obarvana modro. Prikazan je čelni reženj 16 letnega jazbečarja s kognitivno motnjo. Merilo je dolgo 10 µm.

The full article is published in Frontiers in Veterinary Science

Authors: Sonja Prpar Mihevc, Maja Zakošek Pipan, Malan Štrbenc, Boris Rogelj, Gregor Majdič

Anestezija in oksidativni stres pri psih z degeneracijo mitralne zaklopke

lukam — Fri, 23 Oct 2020 08:05:03 +0000

Anaesthesia and oxidative stress in dogs with mitral valve degeneration lukam Fri, 10/23/2020 - 10:14 Small Animal Clinic anaesthesia dogs myxomatous mitral valve disease oxidative stress propofol sevoflurane Research

The influence of propofol and sevoflurane anaesthesia on oxidative stress in dogs with myxomatous mitral valve degeneration.

The influence of propofol and sevoflurane anaesthesia on oxidative stress in dogs with myxomatous mitral valve degeneration

The doctoral dissertation of Katerina Tomsič was set out to evaluate whether anaesthesia with propofol, which is believed to have antioxidant properties, causes less oxidative stress in comparison to anaesthesia with propofol and sevoflurane in dogs in which oxidative stress is present due to the myxomatous mitral valve degeneration (MMVD). Anaesthesia with propofol and sevoflurane resulted in increased oxidative stress in dogs with MMVD in comparison to dogs without MMVD, however both anaesthetic protocols can safely be used in dogs with early stage MMVD.

The results of the research were published in two internationally recognized journals:

Authors

Katerina Tomsič, University of Ljubljana, Veterinary Faculty
Alenka Seliškar, University of Ljubljana, Veterinary Faculty
Alenka Nemec Svete, University of Ljubljana, Veterinary Faculty
Ana Nemec, University of Ljubljana, Veterinary Faculty
Aleksandra Domanjko Petrič, University of Ljubljana, Veterinary Faculty
Tatjana Pirman, University of Ljubljana, Biotechnical Faculty
Vida Rezar, University of Ljubljana, Biotechnical Faculty
Tomaž Vovk, University of Ljubljana, Faculty of Pharmacy

Klinična študija: koencim Q10 in srčne zaklopke pri psih

lukam — Sun, 03 Feb 2019 20:11:39 +0000

Clinical Study: coenzyme Q10 and cardiac valves in dogs lukam Mon, 04/06/2020 - 20:48 Small Animal Clinic Clinics

You are invited to bring your dog for a free examination, which will include laboratory tests. As part of the 3-week study period, you will give your dog a nutritional supplement for two weeks, which you will receive free or charge at the first checkup.

Should you decide to participate in the study, you are invited to bring your dog for a free examination, which will include laboratory tests. As part of the 3-week study period, you will give your dog a nutritional supplement for two weeks, which you will receive free or charge at the first checkup. During the study, a small amount of blood will be removed on four occasions from your dog.

Interested owners are kindly requested to send us an email to: druzhaevanatalia@gmail.com with the title ‘For Coenzyme Q10 Research’. Please provide your contact details, including phone number, and basic information about your dog (as well as any medicine that they are currently receiving). You can specify the time when you would like us to call you.

You can call us on weekdays between 9am and 3pm on 01 477 9315 (Cardiology Clinic of the Small Animal Clinic).

The research is conducted in the Cardiology Clinic of the Clinic for Small Animals under the direction of Associate Professor Dr. Aleksandra Domanjko Petric and Associate Professor Dr. Alenka German Saint.

Izobraževalni priročnik Bolečina brez besed

lukam — Tue, 13 Aug 2019 11:35:05 +0000

‘Pain Without Words’ Handbook lukam Wed, 04/01/2020 - 10:40 Small Animal Clinic Education

The ‘Pain Without Words’ handbook for animal owners and study material for veterinary students was created to help cat and dog owners and veterinarians identify pain in their pets.

It was created as part of the project ‘Attitudes of Slovenian Veterinarians to Pain and Analgesia in Cats and Dogs: An Analysis of the Status and the Introduction of Rating Scales in Clinical Practice’, which took place as part of the public tender ‘The Path to Creative Knowledge 2017-2020’ and was co-funded by The Republic of Slovenia and the European Union from the European Social Fund - link.

The manual is available in hard copy in the library of the Faculty of Veterinary Medicine and the National and University library (NUK). The first print run was made possible by GENERA SI d.o.o., part of Dechra Pharmaceuticals plc. The material is available in electronic form on the websites and Facebook profiles of the Faculty of Veterinary Medicine, the Clinic for Small Animals and the Veterinary Faculty Libraries

‘Pain Without Words’ Handbook (11.0 MB)

Klinična študija: mikoplazme in obolenja pri psih

lukam — Wed, 26 Jun 2019 08:13:20 +0000

Clinical Study: Mycoplasma and Diseases in Dogs lukam Tue, 03/31/2020 - 13:05 Small Animal Clinic Research

As part of its work the Small Animal Clinic is conducting a doctoral study in the field of mycoplasma which cause diseases of the urogenital tract and fertility disorders in dogs.

As part of its work the Small Animal Clinic is conducting a doctoral study in the field of mycoplasma which cause diseases of the urogenital tract and fertility disorders in dogs. Bacteria belonging to the mycoplasma genus are found in different hosts, where they can cause various states of disease, including infertility and diseases of the urogenital tract.

Of the canine mycoplasmas, the most important is Mycoplasma canis (M. canis), which to date has been the most commonly isolated from various parts of the urogenital tract. The purpose of the doctoral thesis is to determine the presence of M. canis in dogs and its role in the development of clinically detectable diseases of the urogenital tract and fertility disorders.

The study is designed as a clinical study in which it would like to include dogs with clinical signs of urinary tract disorders, clinically healthy animals with fertility disorders, and a control group of healthy animals without fertility disorders and signs of urinary tract disease. If you would like to include your dog in the research, it will undergo a free clinical examination, blood and urine collection, and an abdominal ultrasound. When the results of the lab tests are known, we will let you know and discuss if your dog needs treatment or further diagnostics.

Should you have any additional questions, please contact sara.suhadolc@vf.uni-lj.si.

Author: Sara Suhadolc Scholten, DVM

Genetsko preverjanje rodovnikov psov

lukam — Fri, 04 Jan 2019 10:11:09 +0000

Gene Verification of Pedigrees of Dogs lukam Sun, 04/05/2020 - 08:39 Institute of Preclinical Sciences Diagnostics

For several years the Faculty of Veterinary Medicine in Ljubljana has routinely performed genetic checks on the pedigrees of dogs. The method is well-established and integrated into ISAG inter-laboratory controls and can therefore ensure correct and verifiable results. In 2018 the institute signed a contract with the Kennel Club of Slovenia for the production of DNA profiles in dogs. Thus, the institute is certified by the Kennel Club as an official pedigree checker and can offer you and your clients this service which is mandatory for the owners/breeders of dogs.

The institute is certified by the Kennel Club as an official pedigree checker and can offer you and your clients this service which is mandatory for the owners/breeders of dogs.

Instructions for preparation of samples

The test requires DNA that is isolated from the blood, or it may be taken from a swab of the oral mucosa if blood cannot be drawn.

Blood

The blood sample is taken into an EDTA test tube (at least 1ml). The name of the dog, owner and date of blood collection is written on the test tube.

Swab

Swabs are taken by rubbing a cotton bud on the mucous membrane, i.e. the gums or the inside of the cheeks. They are then air dried.

The dried samples are placed in a clean envelope (it must not be polyvinyl and must be air-tight). When sending samples from different dogs, the cotton buds should be stored in different envelopes. It is not recommended to take a swab from puppies under eight weeks old due to the possibility of contamination from the mother.

On the envelope we write down the name of the dog, the owner and the date that the swab was taken are written on the envelope.

Form

When submitting a sample please ensure you also send a completed DNA profile form with information about the dog, owner, payer and veterinarian who took the sample. The form and the sample should be sent by post or courier to the Faculty of Veterinary Medicine, Gerbičeva 60, Ljubljana, and marked ‘for the genetic testing of dogs’.

Gene Testing Form (456.2 KB)

Results are normally available within 10 working days of receipt of the sample.

The cost of producing a DNA profile for an individual dog is:

28,00 € excl. DDV
34,16 € incl. DDV

We can also send you a test tube with EDTA or a kit for taking a sample.

For more information call: 01 4779 120 (Dr. Marko Cotman).

Institute of Preclinical Sciences

Department of Biochemistry, Molecular Biology and Genetics